摘要
目的 通过对2013年72批川贝母药材及饮片全国抽验情况,总结我国川贝母的质量问题,并探讨聚合酶链式反应-限制性内切酶长度多态性方法在检验中应注意的问题。方法 参照《中国药典》2010年版一部标准方法,对所抽取的样品进行检验和研究,发现并探讨其质量问题和检测方法。结果 抽验结果表明,市场上川贝母的不合格率为25.4%,聚合酶链式反应-限制性内切酶长度多态性方法在检验中对取样环节应予以明确规定。结论 应进一步完善和提高标准,加强质量监管,并建立专项抽验的长效机制,保证中药材及饮片的质量和安全。
Abstract
OBJECTIVE To analyze the quality of crude drug materials and decoction pieces of Fritillariae Cirrhosae Bulbus by the national wide quality surveillance of 72 batches of samples in 2013, and study the suitability of polymerase chain reaction-restriction enzymes length polymorphism (PCR-RFLP) tests. METHODS The samples from different origins were identified and detected according to the standard of Chinese Pharmacopoeia. RESULTS Seventeen batches of samples did not meet the requirements, resulting in a disqualification rate of 25.4%. CONCLUSION Further measures should be taken to improve the quality standard and strengthen the quality surveillance of Fritillariae Cirrhosae Bulbus and other species of Chinese material medica.
关键词
川贝母 /
质量分析 /
标准方法
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Key words
Fritillariae Cirrhosae Bulbus /
quality control /
quality standard
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刘薇, 张文娟, 林丽君, 魏锋*, 马双成*.
我国川贝母的质量分析[J]. 中国药学杂志, 2015, 50(4): 305-309 https://doi.org/10.11669/cpj.2015.04.009
LIU Wei,ZHANG Wen-juan,LIN Li-jun,WEI Feng*,MA Shuang-cheng*.
Quality Analysis of Fritillariae Cirrhosae Bulbus[J]. Chinese Pharmaceutical Journal, 2015, 50(4): 305-309 https://doi.org/10.11669/cpj.2015.04.009
中图分类号：
R917
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参考文献
[1] Ch.P (2010) VolⅠ AddⅠ(《中国药典》2010年版. 第一增补本). 2010: 83-84.
[2] HUANG X O, MO K F, HE B Z, et al. Qualitative identification of 5 kinds of Fritillaria .J China Pharm(中国药房),2009, 20(17):1654-1657.
[3] LUO K, MA P, YAO H, et al. Molecular identification of Fritillariae Cirrhosae Bulbus and its adulterants. World Sci Technol(世界科学技术),2012,14(7):1153-1159.
[4] TAN Y, ZHANG L H, LI M C,et al. Identification of Chinese herb Fritillaria cirrrhosa D. Don by DNA fingerprint. Chin Pharm J (中国药学杂志), 2011,46(1): 14-16.
[5] Ch.P(2010) VolⅠ(《中国药典》2010年版. 一部). 2010: Appl 17-18.
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脚注
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基金
国家十二五“重大新药创制”专项(2014ZX09304307-002);国家自然科学基金青年项目(81202909)
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